Histone H1.4 Antibody PA5-117471 from Thermo Fisher Scientific

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WB

Western Blot analysis of Histone H1.4 using Histone H1.4 Polyclonal Antibody (Product # PA5-117471) at 1.48 µg/mL. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 22 kDa. Observed band size: 28 kDa
ICC/IF

Immunocytochemistry/Immunofluorescence analysis of Histone H1.4 in HeLa cells using Histone H1.4 Polyclonal Antibody (Product # PA5-117471) diluted at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supplier Page from
Thermo Fisher Scientific for
Histone H1.4 Antibody

The Histone H1.4 Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to H1-4, and H1.4 linker histone, cluster member. This antibody recognizes Homo Sapiens, Human, and Rat antigen. The Histone H1.4 Antibody has been validated for the following applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.

WB

ICC/IF

Histone H1.4 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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Histone H1.4 Antibody from Thermo Fisher Scientific

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